Description

---

Introduction to genetic, genomic and system analyses for communities.- Genomic analysis of pure cultures and communities.- Protocols for metagenomic library generation and analysis in petroleum hydrocarbon microbe systems.- Preparation and analysis of metatranscriptomic libraries in petroleum hydrocarbon microbe systems.- Prokaryotic metatranscriptomics.- Defining a pipeline for metaproteomic analyses.- Generating enriched metagenomes from active microorganisms with DNA stable isotope probing.- DNA and RNA-based stable isotope probing of hydrocarbon degraders.- Protein stable isotope probing (Protein-SIP).- Protein extraction from contaminated soils and sediments.- Analysis of the regulation of the rate of hydrocarbon and nutrient flow through microbial communities.- Physiological modelling of complex bacterial communities.- Protocol for evaluating the permissiveness of bacterial communities towards conjugal plasmids by quantification and isolation of transconjugants.